anti psmb7  (Boster Bio)

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: (A) PSMB7 is highly expressed in many solid tumors, including LUAD tissues (B, C). The ROC curve area was 0.712 (D), indicating that PSMB7 may serve as a diagnostic biomarker for LUAD. P -values were calculated using a two-tailed unpaired Student’s t -test, with ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. LUAD, lung adenocarcinoma; PSMB7, proteasome subunit beta type-7; ROC curve, Receiver Operating Characteristic curve.

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques: Diagnostic Assay, Biomarker Discovery, Two Tailed Test

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: RT-qPCR (A) The results showed that the PSMB7 level in LUAD tissues was higher than that in adjacent tissues. (B and C) PSMB7 protein expression levels from the Human Protein Atlas database. (D and E) The differential expression of PSMB7 protein was verified by immunohistochemistry. LUAD, lung adenocarcinoma; IHC, immunohistochemistry; RT-qPCR, Reverse Transcription Quantitative Polymerase Chain Reaction; HPA, Human Protein Atlas. Representative RT-qPCR and IHC results in 15 samples. (Comment: expanded by Zhu Guihua).

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Quantitative Proteomics, Immunohistochemistry, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: (A) OS and (B) DSS in patients with lung adenocarcinoma with high and low PSMB7 expression levels. (C) Forest plot depicting OS outcomes in patients with lung adenocarcinoma based on multivariate Cox regression analysis. DSS, disease-specific survival; OS, overall survival.

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques: Expressing

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: (A–I) OS curves of stage I and III , stage II and III , T1 and T3, T1 and T4, N0 and N2, M0 and M1, PD and CR, smoker, age, between high- and low- PSMB7 patients with LUAD. LUAD, lung adenocarcinoma; OS, overall survival; PD, Progressive Disease; CR, Complete Response.

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques:

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: (A) Blue and red dots indicate significantly downregulated and upregulated DEGs in the volcano plot, respectively. (B) The top 10 DEGs were positively correlated with PSMB7 levels. (C) KEGG, GO, and GSEA. (D) Analyses of DEGs. DEG, differentially expressed gene; GO, Gene Ontology; GSEA, gene set enrichment analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes. DEGs, Differentially Expressed Genes.

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques:

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: (A) Promoter methylation level of PSMB7 in LUAD was significantly lower than that in normal lung tissue. (B) The correlation between PSMB7 mRNA expression and promoter methylation. (C) Kaplan–Meier survival curves for different PSMB7 methylation sites. P -values were calculated using a two-tailed unpaired Student’s t -test. LUAD, lung adenocarcinoma.

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques: Methylation, Expressing, Two Tailed Test

Journal: PeerJ

Article Title: Identification and validation of PSMB7 as a novel biomarker for prognosis and immune infiltrates of lung adenocarcinoma

doi: 10.7717/peerj.19958

Figure Lengend Snippet: (A) Correlation between PSMB7 expression and 24 types of immune cells. (B–E) Comparison of immune infiltration levels of immune cells (including Tem, TFH, B cells and Mast cells) between high and low PSMB7 level groups. (F–I) The expression of PSMB7 was negatively correlated with the level of infiltrating immune cells, including Tem, TFH, B cells and Mast cells. P -values were calculated with two-tailed unpaired Student’s t -test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. LUAD, lung adenocarcinoma; Tem, effector T memory cell; TFH, follicular T helper cell.

Article Snippet: The sections were stained with an anti-human PSMB7 antibody (1:2000; mouse; TA504368S; OriGene, Wuxi, China), following the manufacturer’s instructions.

Techniques: Expressing, Comparison, Two Tailed Test